Download Analytical Techniques In DNA Sequencing by Brian K. Nunnally PDF

By Brian K. Nunnally

Whereas one marvels on the DNA generated by way of the Human Genome venture, maybe simply as major and interesting is the advance of the fantastic analytical know-how that has accepted us access into the genomic period and a complete new point of clinical knowing.

Analytical ideas in DNA Sequencing takes a glance on the a number of DNA sequencing concepts that have been constructed and placed to exploit in the course of and after the Human Genome venture. Nunnally makes a unique attempt to track the improvement of this department of analysis because the early days of radioisotope sequencing. designated recognition is paid to the step forward Sanger process (1977), in addition to to a number of different ideas that better the accuracy, better the detection limits, and in quite a few methods dramatically diminished the time had to generate a DNA series.

This accomplished quantity describes the advancements in DNA separation in response to complicated capillary electrophoresis and microchip sequencing. It additionally seems to be on the use of mass spectrometry and unmarried molecule detection, and takes an in-depth examine using quite a few fluorescence equipment for the detection of sequencing fragments. One bankruptcy is devoted to chip-based microfabricated sequencing structures, that can good be the way forward for DNA sequencing.

The book’s ten chapters, authored by means of quite a few major specialists within the box, comprise some of the far-reaching purposes of DNA sequencing. complete chapters are devoted to DNA typing for forensics, a reevaluation of old DNA, and genome research.

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SC Hung, RA Mathies, AN Glazer. Optimization of spectroscopic and electrophoretic properties of energy transfer primers. Anal Biochem 252:78–88, 1997. 7. item=6479. 8. SC Hung, RA Mathies, AN Glazer. Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations. Anal Biochem 255:32–38, 1998. 9. LG Lee, SL Spurgeon, CR Heiner, SC Benson, BB Rosenblum, SM Menchen, RJ Graham, A Constantinescu, KG Upadhya, JM Cassel. New energy transfer dyes for DNA sequencing. Nucleic Acids Res 25:2816–2822, 1997.

J Chromatogr B 695:85–92, 1997. 22. JR Lackowicz. Principles of Fluorescence Spectroscopy. New York: Plenum Press, 1983, 52–53. 23. L Li, LB McGown. Effects of gel material on fluorescence lifetime detection of yes and dye-labeled DNA primers in capillary electrophoresis. J Chromatogr A 841:95–103, 1999. 24. SH Mihindukulasuriya, TK Morcone, LB McGown. Characterization of acridone dyes for use in four-decay detection in DNA sequencing. Electrophoresis 24:20–25, 2003. 3 Microscale Sample Preparation for DNA Sequencing and Genotyping Yonghua Zhang and Edward S.

3 Microscale Sample Preparation for DNA Sequencing and Genotyping Yonghua Zhang and Edward S. 56 INTRODUCTION The greatest achievement in molecular biology in the past decade is undoubtedly the sequencing of the human genome. The announcement of the completion of the Human Genome Project in June 2000 marks the availability of the rough draft about 3 years ahead of schedule. The remaining sequencing tasks should be completed in 2003. There is general agreement that one of the major developments that allowed such rapid progress is the availability of high-throughput DNA 29 30 Analytical Techniques in DNA Sequencing sequencers based on capillary electrophoresis (CE).

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