By Rajni Hatti-Kaul
A mix of polymers, or one polymer and a salt, in an aqueous medium separates into levels: this phenomenon turns out to be useful in biotechn- ogy for product separations. Separation of organic molecules and debris in those aqueous two-phase platforms (ATPS) used to be initiated over forty years in the past by means of P.-Å. Albertsson, and later proved to be of mammoth software in biochemical and mobile organic examine. a lift within the program of ATPS was once visible while difficulties of separations in biotechnology approaches have been encountered. Its simplicity, biocompatibility, and amenability to effortless scaleup operations make using ATPS very beautiful for large-scale bioseparations. regardless of the benefits ATPS enjoys over different separation strategies, the applying of two-phase platforms has for a very long time been restrained to chose labora- ries. fresh years have, even though, proven a development within which expanding numbers of researchers hire two-phase partitioning suggestions in either uncomplicated and utilized examine.
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Additional info for Aqueous two-phase systems. Methods and protocols
Applications in Cell Biology and Biotechnology. Plenum, New York. 7. -O. (1989) Influence of disrupted biomass on the partitioning of `-galactosidase fused protein A. Enzyme Microb. Technol. 11, 730–735. Preparation of ATPS 23 3 Preparation of Aqueous Two-Phase Systems Daniel Forciniti 1. Introduction The most common aqueous two-phase systems (ATPS) are created using either two incompatible polymers or one polymer and a salt (1). These mixtures separate into two liquid phases which are in equilibrium; each phase is enriched in one or the other of the respective phase forming components.
The required software is described in detail in (3). The program is easy to run and quickly converges to provide values for the interfacial tension and provides as well the contact angle, radius of curvature at the origin, surface area, drop volume and contact radius. We have found that the values for a should be averaged over at least 12 independent drop profiles to provide reliable answers. For a typical aqueous two phase system the values generally have standard deviations corresponding to uncertainties of ±4%.
2. illustrates turbidometric titration where a series of systems (᭹) are prepared and titrated until a one-phase system is formed—the binodal lies just above this point (᭡). 3. illustrates the determination of nodes () for systems, which is accomplished by preparing a series of systems lying on different tie-lines (------᭹------) and analyzing the concentration of components in the top and bottom phase. 3. Node Determination 1. Prepare a series of systems and analyze the phase composition of the top and bottom phase.